The effects of transplanted adipose-derived multipotent mesenchymal stromal cells from mice of different age or from aging donors in combination with melatonin at experimental parkinsonism
نویسندگان
چکیده
The transplantation of adipose-derived multipotent mesenchymal stromal cells (ADSCs) in Parkinson’s disease/parkinsonism is a promising area their therapy. effects such may be influenced by the age donor and biologically active factors. purpose study to compare effect transplanted ADSCs mice different on parameters behaviour, oxidative stress neuroinflammation brain with an experimental model parkinsonism; evaluate changes from older donors under influence exogenous hormone melatonin. Materials methods. object was adult (5-6 months) aging (15-17 129/Sv mice. Adult were injected once neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), after 17 days – or at dose 700 thousand tail vein. Some received combination Behavioural assessed open-field, rigidity rotarod tests; relative content macrophages measured brain, malondialdehyde (MDA), activity antioxidant enzymes. Results. Under MPTP, number squares, rearings, body length significantly less than intact group, muscle tone higher; MDA increases superoxide dismutase (SOD) decreases. After ADSCs, step increase significantly, but not level mice; SOD, glutathione reductase (GR) proportion brain. administration donors, values behavioural correspond control group (only MPTP), SOD corresponds animals. In treated melatonin, direction parameters, GR activity, macrophage percentage similar that observed ADSCs. Conclusions. MPTP parkinsonism depend are more pronounced derived melatonin consistent those donors.
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ژورنال
عنوان ژورنال: ???????? ?? ??????? ????????????????
سال: 2022
ISSN: ['2308-3794', '2311-021X']
DOI: https://doi.org/10.22494/cot.v10i1.134